Typically equipped with a 24-place fixed-angle rotor (e.g., F-45-24-11) for 1.5/2.0 mL tubes.
The Eppendorf Centrifuge 5414 is a tabletop centrifuge that combines high speed with a compact design. Its robust construction and low-maintenance design make it an ideal choice for laboratories with limited space. With a maximum speed of 14,000 rpm and a range of rotor options, the Centrifuge 5414 can handle a wide variety of samples, from microcentrifuge tubes to PCR strips. eppendorf centrifuge 5414 manual
The original 5414 manual dedicates a full chapter to user-level maintenance. Adhere to these intervals: Typically equipped with a 24-place fixed-angle rotor (e
If you have recently inherited a 5414 or are trying to revive one, locating the original is critical for safe operation and accurate rotor speed calibration. This article serves as a comprehensive resource, consolidating operational instructions, maintenance protocols, safety warnings, and troubleshooting tips derived from the original user manual. With a maximum speed of 14,000 rpm and
The 5414 uses two rotary knobs. There is no digital display.
In the history of molecular biology, few pieces of benchtop equipment have achieved the iconic status of the Eppendorf Centrifuge 5414. Before the era of high-speed, refrigerated microcentrifuges with digital displays, the 5414 was a workhorse in labs worldwide. While this model is now considered a classic or "vintage" instrument (largely superseded by the 5415, 5417, and 5418 series), thousands of these units are still in active use in teaching labs, field stations, and small research facilities.