Primer3 Input -version 0.4.0- __full__ — Full & Top-Rated

Relax PRIMER_TM_MIN , PRIMER_GC_MIN , or expand PRIMER_PRODUCT_SIZE_RANGE .

The power of Primer3 lies in its thermodynamic models. Version 0.4.0 uses the SantaLucia 1998 nearest-neighbor parameters by default. primer3 input -version 0.4.0-

primer3 < my_input.txt > my_output.txt