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: 250-mL wide-mouth Erlenmeyer flasks and a magnetic stirrer with an inert plastic-coated bar. Reagents : Indicators : Bromphenol blue and bromcresol green. Solvents : Chloroform and isopropyl alcohol. Chemicals : Salicylaldehyde and phenyl isothiocyanate.
Accurately weigh a sample (typically 0.2–0.5 grams) and dissolve in a mixture of glacial acetic acid and chlorobenzene. astm d2074 pdf
ASTM D2074 is the Standard Test Method for the Analysis of Fatty Acid Methyl Esters (FAME) by Gas Chromatography. It is a standard developed by ASTM International, formerly known as the American Society for Testing and Materials. : 250-mL wide-mouth Erlenmeyer flasks and a magnetic
: The values are typically calculated using the formula: is the volume of HCl used (mL), is the normality of the HCl, and is the specimen weight (g). Required Apparatus and Reagents Chemicals : Salicylaldehyde and phenyl isothiocyanate
In the food industry, understanding the fatty acid profile is essential for nutritional labeling. Manufacturers need to quantify saturated, monounsaturated, and polyunsaturated fats. While other methods like AOAC 996.06 are often cited for nutrition labels, ASTM D2074 provides a robust alternative for raw material verification.
For those utilizing the standard, the outlines a specific workflow. While the full details are within
The relevance of ASTM D2074 extends across multiple industries. When a chemist searches for an , they are often trying to solve a critical quality control issue.