A: No. GraphPad officially ended support for version 4.0 in 2010. Their knowledge base may still have legacy articles, but no live support.
If the software installs but refuses to launch, you must utilize the Compatibility Mode feature introduced in Vista. graphpad prism 4.0 vista
The software is designed to produce publication-quality visuals directly from raw data: graphpad prism 4.0 vista
When users attempted to install the older Prism 4.0 on the newer Vista platform, they encountered a clash of philosophies: an application designed for a permissive environment running on a restrictive, security-heavy operating system. graphpad prism 4.0 vista
A: Partially. The installer is 32-bit and runs under WoW64. However, 64-bit Vista handles legacy registry calls poorly. Expect occasional crashes when saving .PZF files.