No. The G31 chipset has a hardware limitation of 4GB max (only ~3.2GB usable in 32-bit OS).
Place the AFUDOS.exe utility and the .ROM BIOS file (e.g., 100225S.ROM ) into the root of the USB drive. ecs g31t-m7 bios update
Always use the official ECS Download Center to find the correct ROM for your specific version. ecs g31t-m7 bios update