Primer Premier 6 ((hot))

Users can design primers for up to 10-plex assays by launching batch searches that check for cross-reactivity between all primers in the pool.

Designing one primer pair is easy. Designing 10 that work in the same tube is hard. PP6 includes a that designs sets of primers with similar Tm values that will not cross-react with each other. primer premier 6

Need help troubleshooting a primer-dimer issue? Run your sequence through PP6’s "Cross-Dimer" analysis and look for a ΔG below -6.0 kcal/mol. Users can design primers for up to 10-plex

), ensuring primers are free from dimers, repeats, and runs. SNP Genotyping: ensuring primers are free from dimers